Diet Rodent Model Body Weight Fasting Plasma Glucose / Insulin Steatosis Steato Hepatitis Fibrosis Time Frame (Fibrosis)# Methionine Choline Deficient Diet (MCD) Rats and Mice ↓ ↓ ╋ ╋ ╋ 4-8 weeks 0.1% Methionine Choline Deficient High-Fat Diet (CDAHFD) Mainly Mice ↓* No Change ╋ ╋ ╋ 6-12 weeks Choline Deficient Amino Acid Diet (CDAA) Rats and Mice No Change ↑ Mainly Mice ╋ ╋ ╋ 4-8 weeks (rats) 12 weeks (mice) Choline Deficient High-Fat Diet (CD) Mainly Mice ↑ ↑ ╋ ╋ ╋ 12 months High-Fat Diet (HFD) Rats and Mice ↑ ↑ ╋ ╋ ╋ (MILD) 24 weeks (rats) 16 weeks (mice) High Fructose Diet (HFR) Mainly Mice No Change ↑ ╋ ╋ ╋ 12 weeks High Fat, High Fructose, High Cholesteol Diet Rats and Mice ↑ ↑ ╋ ╋ ╋ Mainly Mice 20-30 weeks (mice)

1. +, mild ; ++, modest ; +++, severe. Body weight and fasting glucose/insulin for arrows is relative to the control diet. 2. The length depends on diet formula ; length of the study ; and species, strain, and gender of the animal model. * Compared with a low-fat, metionine- and choline- sufficient group. Body weight of these animals typically remains unchanged compared with baseline.